RESEARCH FROM SOME OF OUR RECENT MANUSCRIPTS:
Penke LR, Speth JM, Huang SK, Fortier SM, Baas J, Peters-Golden M. KLF4 is a therapeutically tractable brake on fibroblast activation that promotes resolution of pulmonary fibrosis. JCI Insight. 2022 Aug 22;7(16):e160688. doi: 10.1172/jci.insight.160688. PMID: 35852857; PMCID: PMC9462506.
Caporarello N, Lee J, Pham TX, Jones DL, Guan J, Link PA, Meridew JA, Marden G, Yamashita T, Osborne CA, Bhagwate AV, Huang SK, Nicosia RF, Tschumperlin DJ, Trojanowska M, Ligresti G. Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis. Nat Commun. 2022 Jul 25;13(1):4170. doi: 10.1038/s41467-022-31890-4. Erratum in: Nat Commun. 2022 Sep 28;13(1):5687. PMID: 35879310; PMCID: PMC9314350.
Pham TX, Lee J, Guan J, Caporarello N, Meridew JA, Jones DL, Tan Q, Huang SK, Tschumperlin DJ, Ligresti G. Transcriptional analysis of lung fibroblasts identifies PIM1 signaling as a driver of aging-associated persistent fibrosis. JCI Insight. 2022 Mar 22;7(6):e153672. doi: 10.1172/jci.insight.153672. PMID: 35167499; PMCID: PMC8986080.
Ting C, Aspal M, Vaishampayan N, Huang SK, Riemondy KA, Wang F, Farver C, Zemans RL. Fatal COVID-19 and Non-COVID-19 Acute Respiratory Distress Syndrome Is Associated with Incomplete Alveolar Type 1 Epithelial Cell Differentiation from the Transitional State without Fibrosis. Am J Pathol. 2022 Mar;192(3):454-467. doi: 10.1016/j.ajpath.2021.11.014. Epub 2021 Dec 30. PMID: 34973949; PMCID: PMC8730538.
Qian G, Adeyanju O, Sunil C, Huang SK, Chen SY, Tucker TA, Idell S, Guo X. Dedicator of Cytokinesis 2 (DOCK2) Deficiency Attenuates Lung Injury Associated with Chronic High-Fat and High-Fructose Diet-Induced Obesity. Am J Pathol. 2022 Feb;192(2):226-238. doi: 10.1016/j.ajpath.2021.10.011. Epub 2021 Nov 10. PMID: 34767813; PMCID: PMC8883439.
Gajjala PR, Kasam RK, Soundararajan D, Sinner D, Huang SK, Jegga AG, Madala SK. Dysregulated overexpression of Sox9 induces fibroblast activation in pulmonary fibrosis. JCI Insight. 2021 Oct 22;6(20):e152503. doi: 10.1172/jci.insight.152503. PMID: 34520400; PMCID: PMC8564901.
Zhu Y, Chang J, Tan K, Huang SK, Liu X, Wang X, Cao M, Zhang H, Li S, Duan X, Chang Y, Fan Y, Cao P. Clioquinol Attenuates Pulmonary Fibrosis through Inactivation of Fibroblasts via Iron Chelation. Am J Respir Cell Mol Biol. 2021 Aug;65(2):189-200. doi: 10.1165/rcmb.2020-0279OC. PMID: 33861690.
Al-Habeeb F, Aloufi N, Traboulsi H, Liu X, Nair P, Haston C, Azuelos I, Huang SK, White ES, Gallouzi IE, Di Marco S, Eidelman DH, Baglole CJ. Human antigen R promotes lung fibroblast differentiation to myofibroblasts and increases extracellular matrix production. J Cell Physiol. 2021 Oct;236(10):6836-6851. doi: 10.1002/jcp.30380. Epub 2021 Apr 14. PMID: 33855709; PMCID: PMC8390431.
Huang SK, Tripathi P, Koneva LA, Cavalcante RG, Craig N, Scruggs AM, Sartor MA, Deng F, Chen Y. Effect of concentration and duration of particulate matter exposure on the transcriptome and DNA methylome of bronchial epithelial cells. Environ Epigenet. 2021 Feb 28;7(1):dvaa022. doi: 10.1093/eep/dvaa022. PMID: 33692908; PMCID: PMC7928203.
Huang SK. Knocking Out Smoking and Pulmonary Hypertension with a K. Am J Respir Crit Care Med. 2021 May 15;203(10):1216-1218. doi: 10.1164/rccm.202011-4121ED. PMID: 33357112; PMCID: PMC8456471.
Aloufi N, Traboulsi H, Ding J, Fonseca GJ, Nair P, Huang SK, Hussain SNA, Eidelman DH, Baglole CJ. Angiotensin-converting enzyme 2 expression in COPD and IPF fibroblasts: the forgotten cell in COVID-19. Am J Physiol Lung Cell Mol Physiol. 2021 Jan 1;320(1):L152-L157. doi: 10.1152/ajplung.00455.2020. Epub 2020 Oct 28. PMID: 33112187; PMCID: PMC7869954.
Kasam RK, Ghandikota S, Soundararajan D, Reddy GB, Huang SK, Jegga AG, Madala SK. Inhibition of Aurora Kinase B attenuates fibroblast activation and pulmonary fibrosis. EMBO Mol Med. 2020 Sep 7;12(9):e12131. doi: 10.15252/emmm.202012131. Epub 2020 Aug 6. PMID: 32761869; PMCID: PMC7507328.
Caporarello N, Meridew JA, Aravamudhan A, Jones DL, Austin SA, Pham TX, Haak AJ, Moo Choi K, Tan Q, Haresi A, Huang SK, Katusic ZS, Tschumperlin DJ, Ligresti G. Vascular dysfunction in aged mice contributes to persistent lung fibrosis. Aging Cell. 2020 Aug;19(8):e13196. doi: 10.1111/acel.13196. Epub 2020 Jul 21. PMID: 32691484; PMCID: PMC7431829.
Podolsky MJ, Yang CD, Valenzuela CL, Datta R, Huang SK, Nishimura SL, Dallas SL, Wolters PJ, Le Saux CJ, Atabai K. Age-dependent regulation of cell-mediated collagen turnover. JCI Insight. 2020 May 21;5(10):e137519. doi: 10.1172/jci.insight.137519. PMID: 32315288; PMCID: PMC7259530.
Huang SK. A Fresh Take on the “TCA” Cycle: TETs, Citrate, and Asthma. Am J Respir Cell Mol Biol. 2020 Jul;63(1):1-3. doi: 10.1165/rcmb.2020-0101ED. PMID: 32223718; PMCID: PMC7328252.
Penke LR, Ouchi H, Speth JM, Lugogo N, Huang YJ, Huang SK, Peters-Golden M. Transcriptional regulation of the IL-13Rα2 gene in human lung fibroblasts. Sci Rep. 2020 Jan 23;10(1):1083. doi: 10.1038/s41598-020-57972-1. PMID: 31974428; PMCID: PMC6978327.
Scruggs AM, Grabauskas G, Huang SK. The Role of KCNMB1 and BK Channels in Myofibroblast Differentiation and Pulmonary Fibrosis. Am J Respir Cell Mol Biol. 2020 Feb;62(2):191-203. doi: 10.1165/rcmb.2019-0163OC. PMID: 31486669; PMCID: PMC6993546.
Tripathi P, Deng F, Scruggs AM, Chen Y, Huang SK. Variation in doses and duration of particulate matter exposure in bronchial epithelial cells results in upregulation of different genes associated with airway disorders. Toxicol In Vitro. 2018 Sep;51:95-105. doi: 10.1016/j.tiv.2018.05.004. Epub 2018 May 9. PMID: 29753051; PMCID: PMC6464127.
Dodi AE, Ajayi IO, Chang C, Beard M, Ashley SL, Huang SK, Thannickal VJ, Tschumperlin DJ, Sisson TH, Horowitz JC. Regulation of fibroblast Fas expression by soluble and mechanical pro-fibrotic stimuli. Respir Res. 2018 May 10;19(1):91. doi: 10.1186/s12931-018-0801-4. PMID: 29747634; PMCID: PMC5946418.
Scruggs AM, Koh HB, Tripathi P, Leeper NJ, White ES, Huang SK. Loss of CDKN2B Promotes Fibrosis via Increased Fibroblast Differentiation Rather Than Proliferation. Am J Respir Cell Mol Biol. 2018 Feb 8. PMID: 29420051
Sanders YY, Liu H, Scruggs AM, Duncan SR, Huang SK, Thannickal VJ. Epigenetic Regulation of Caveolin-1 Gene Expression in Lung Fibroblasts. Am J Respir Cell Mol Biol. 2017 Jan;56(1):50-61. doi: 10.1165/rcmb.2016-0034OC. PMID: 27560128; PMCID: PMC5248956.
Koh HB, Scruggs AM, Huang SK. Transforming growth factor-β1 increases DNA methyltransferase 1 and -3a expression through distinct post-transcriptional mechanisms in lung fibroblasts. J Biol Chem. 2016 Sep 9;291(37):19287-98. PMID: 27405758; PMCID: PMC5016670
Zasłona Z, Scruggs AM, Peters-Golden M, Huang SK. Protein kinase A inhibition of macrophage maturation is accompanied by an increase in DNA methylation of the colony stimulating factor 1 receptor gene. Immunology. 2016 Oct;149(2):225-37. PMID: 27353657; PMCID: PMC501168
Wettlaufer SH, Scott JP, McEachin RC, Peters-Golden M, Huang SK. Reversal of the transcriptome by prostaglandin E2 during myofibroblast dedifferentiation. Am J Respir Cell Mol Biol. 2016 Jan;54(1):114-27. PMID: 26098591; PMCID: PMC4742926
Neagos J, Standiford TJ, Newstead MW, Zeng X, Huang SK, Ballinger MN. Epigenetic Regulation of Tolerance to Toll-Like Receptor Ligands in Alveolar Epithelial Cells. Am J Respir Cell Mol Biol. 2015 Dec;53(6):872-81. doi: 10.1165/rcmb.2015-0057OC. PMID: 25965198; PMCID: PMC4742943.
Huang SK, Peters-Golden M. Prostaglandin E₂ and polyenylphosphatidylcholine: stiff competition for the fibrotic complications of inflammatory bowel disease? Dig Dis Sci. 2015 Jun;60(6):1514-6. doi: 10.1007/s10620-015-3668-y. Epub 2015 Apr 24. PMID: 25902749; PMCID: PMC4830265.
Domingo-Gonzalez R, Wilke CA, Huang SK, Laouar Y, Brown JP, Freeman CM, Curtis JL, Yanik GA, Moore BB. Transforming growth factor-β induces microRNA-29b to promote murine alveolar macrophage dysfunction after bone marrow transplantation. Am J Physiol Lung Cell Mol Physiol. 2015 Jan 1;308(1):L86-95. PMID: 25361568; PMCID: PMC4281703
Huang SK, Horowitz JC. Outstaying their welcome: the persistent myofibroblast in IPF. Austin J Pulm Respir Med. 2014; 1(1):3. PMID: 25309962; PMCID: PMC4189780
Huang SK, Scruggs AM, McEachin RC, White ES, Peters-Golden M. Lung fibroblasts from patients with idiopathic pulmonary fibrosis exhibit genome-wide differences in DNA methylation compared to fibroblasts from nonfibrotic lung. PLoS ONE. 2014 Sep 12;9(9):e107055. PMID: 25215577; PMCID: PMC4162578
Penke LR, Huang SK, White ES, Peters-Golden M. Prostaglandin E2 inhibits α-smooth muscle actin transcription during myofibroblast differentiation via distinct mechanisms of modulation of serum response factor and myocardin-related transcription factor-A. J Biol Chem. 2014 Jun 13;289(24):17151-17162. PMID: 24802754; PMCID: PMC4059156
Rosenberg AA, Haft JW, Bartlett R, Iwashyna TJ, Huang SK, Lynch WR, Napolitano LM. Prolonged duration ECMO for ARDS: futility, native lung recovery, or transplantation? ASAIO J. 2013 Nov-Dec;59(6):642-50. doi: 10.1097/MAT.0b013e3182a9e341. PMID: 24172270.
Ajayi IO, Sisson TH, Higgins PD, Booth AJ, Sagana RL, Huang SK, White ES, King JE, Moore BB, Horowitz JC. X-linked inhibitor of apoptosis regulates lung fibroblast resistance to Fas-mediated apoptosis. Am J Respir Cell Mol Biol. 2013 Jul;49(1):86-95. doi: 10.1165/rcmb.2012-0224OC. PMID: 23492187; PMCID: PMC3727886.
Garrison G, Huang SK, Okunishi K, Scott JP, Penke LR, Scruggs AM, Peters-Golden M. Reversal of myofibroblast differentiation by prostaglandin E2. Am J Respir Cell Mol Biol. 2013 May;48(5):550-8. PMID: 23470625; PMCID: PMC3707380.
Huang SK, Scruggs AM, Donaghy J, Horowitz JC, Zaslona Z, Przybranowski S, White ES, Peters-Golden M. Histone modifications are responsible for decreased Fas expression and apoptosis resistance in fibrotic lung fibroblasts. Cell Death Dis. 2013 May 2; 4:e621. PMID: 23640463; PMCID: PMC3674355
Sisson TH, Maher TM, Ajayi IO, King JE, Higgins PD, Booth AJ, Sagana RL, Huang SK, White ES, Moore BB, Horowitz JC. Increased survivin expression contributes to apoptosis-resistance in IPF fibroblasts. Adv Biosci Biotechnol. 2012 Oct;3(6A):657-664. doi: 10.4236/abb.2012.326085. PMID: 23355956; PMCID: PMC3553664.
Domingo-Gonzalez R, Huang SK, Laouar Y, Wilke CA, Moore BB. COX-2 expression is upregulated by DNA hypomethylation after hematopoietic stem cell transplantation. J Immunol. 2012 Nov 1;189(9):4528-36. doi: 10.4049/jimmunol.1201116. Epub 2012 Sep 24. PMID: 23008450; PMCID: PMC3478470.
Huang SK, Scruggs AM, Donaghy J, McEachin RC, Fisher AS, Richardson BC, Peters-Golden M. Prostaglandin E2 increases fibroblast gene-specific and global DNA methylation via increased DNA methyltransferase expression. FASEB J. 2012 Sep;26(9):3703-14. PMID: 22645246; PMCID: PMC3425823
Horowitz JC, Ajayi IO, Kulasekaran P, Rogers DS, White JB, Townsend SK, White ES, Nho RS, Higgins PD, Huang SK, Sisson TH. Survivin expression induced by endothelin-1 promotes myofibroblast resistance to apoptosis. Int J Biochem Cell Biol. 2012 Jan;44(1):158-69. doi: 10.1016/j.biocel.2011.10.011. Epub 2011 Oct 25. PMID: 22041029; PMCID: PMC3241828.
Okunishi K, Sisson TH, Huang SK, Hogaboam CM, Simon RH, Peters-Golden M. Plasmin overcomes resistance to prostaglandin E2 in fibrotic lung fibroblasts by reorganizing protein kinase A signaling. J Biol Chem. 2011 Sep 16;286(37):32231-43. PMID: 21795691; PMCID: PMC3173171
Huang SK, Fisher AS, Scruggs AM, White ES, Hogaboam CM, Richardson BC, Peters-Golden M. Hypermethylation of PTGER2 confers prostaglandin E2 resistance in fibrotic fibroblasts from humans and mice. Am J Pathol. 2010 Nov;177(5):2245-55. PMID: 20889571; PMCID: PMC2966784
Bauman KA, Wettlaufer SH, Okunishi K, Vannella KM, Stoolman JS, Huang SK, Courey AJ, White ES, Hogaboam CM, Simon RH, Toews GB, Sisson TH, Moore BB, Peters-Golden M. The antifibrotic effects of plasminogen activation occur via prostaglandin E2 synthesis in humans and mice. J Clin Invest. 2010 Jun;120(6):1950-60. doi: 10.1172/JCI38369. Epub 2010 May 24. PMID: 20501949; PMCID: PMC2877926.
Sagana RL, Yan M, Cornett AM, Tsui JL, Stephenson DA, Huang SK, Moore BB, Ballinger MN, Melonakos J, Kontos CD, Aronoff DM, Peters-Golden M, White ES. Phosphatase and tensin homologue on chromosome 10 (PTEN) directs prostaglandin E2-mediated fibroblast responses via regulation of E prostanoid 2 receptor expression. J Biol Chem. 2009 Nov 20;284(47):32264-71. doi: 10.1074/jbc.M109.004796. Epub 2009 Oct 6. PMID: 19808686; PMCID: PMC2781639.
Huang SK, White ES, Wettlaufer SH, Grifka H, Hogaboam CM, Thannickal VJ, Horowitz JC, Peters-Golden M. Prostaglandin E2 induces fibroblast apoptosis by modulating multiple survival pathways. FASEB J. 2009 Dec;23(12):4317-26. PMID: 19671668; PMCID: PMC2812040
Hao Y, Senn T, Opp JS, Young VB, Thiele T, Srinivas G, Huang SK, Aronoff DM. Lethal toxin is a critical determinant of rapid mortality in rodent models of Clostridium sordellii endometritis. Anaerobe. 2010 Apr;16(2):155-60. doi: 10.1016/j.anaerobe.2009.06.002. Epub 2009 Jun 13. PMID: 19527792; PMCID: PMC2856776.
Chung J, Serezani CH, Huang SK, Stern JN, Keskin DB, Jagirdar R, Brock TG, Aronoff DM, Peters-Golden M. Rap1 activation is required for Fc gamma receptor-dependent phagocytosis. J Immunol. 2008 Oct 15;181(8):5501-9. doi: 10.4049/jimmunol.181.8.5501. PMID: 18832707; PMCID: PMC3077557.
Huang SK, Peters-Golden M. Eicosanoid lipid mediators in fibrotic lung diseases: ready for prime time? Chest. 2008 Jun;133(6):1442-1450. doi: 10.1378/chest.08-0306. PMID: 18574287; PMCID: PMC2582216.
Huang SK, Wettlaufer SH, Chung J, Peters-Golden M. Prostaglandin E2 inhibits specific lung fibroblast functions via selective actions of PKA and Epac-1. Am J Respir Cell Mol Biol. 2008 Oct;39(4):482-9. PMID: 18421013; PMCID: PMC2551707
Huang SK, Myers JL, Flaherty KR. The role of lung biopsy in the diagnosis and management of idiopathic interstitial pneumonia. Expert Opin Med Diagn. 2008 Feb;2(2):183-90. doi: 10.1517/17530059.2.2.183. PMID: 23485138.
Huang SK, Wettlaufer SH, Hogaboam CM, Flaherty KR, Martinez FJ, Myers JL, Colby TV, Travis WD, Toews GB, Peters-Golden M. Variable prostaglandin E2 resistance in fibroblasts from patients with usual interstitial pneumonia. Am J Resp Crit Care Med. 2008 Jan 1;177(1):66-74. PMID: 17916807; PMCID: PMC2176116